Development of microsatellite markers for the Japanese orange fly, Bactrocera tsuneonis (Diptera: Tephritidae)

To monitor and control the Japanese orange fly Bactrocera tsuneonis (Miyake) (Diptera: Tephritidae), it is important to determine their dispersal ability. Therefore, genetic structure of this species, we developed microsatellite markers at 17 loci for B. tsuneonis. A total 80 adult females males randomly collected from abandoned orchards in Oita Yamaguchi Prefectures, western part Japan, were used polymorphism analysis using markers. The average number alleles was 2.65 loci, which two did not show polymorphism. expected heterozygosity ranged 0 0.663, although observed relatively low between 0.105. null allele frequencies varied among populations. Moreover, no found three larval populations Prefecture. These results suggested a high degree inbreeding species. Significant differentiation same population observed, bottleneck effect detected but Oita, congruent with history an expanding distribution could be discrimination expand geographic potentially contributing pest management